dc.contributor.author | Kataya, Amr Ramzy Abass | |
dc.contributor.author | ElShobaky, Ahmed | |
dc.contributor.author | Heidariahootapeh, Behzad | |
dc.contributor.author | Nemie-Feyissa, Dugassa | |
dc.contributor.author | Thelen, Jay J. | |
dc.contributor.author | Lillo, Cathrine | |
dc.date.accessioned | 2021-05-11T14:01:06Z | |
dc.date.available | 2021-05-11T14:01:06Z | |
dc.date.created | 2021-02-02T17:22:32Z | |
dc.date.issued | 2020-04 | |
dc.identifier.citation | Kataya, A.R.A., Elshobaky, A., Heidari, B. et al. (2020) Multi-targeted trehalose-6-phosphate phosphatase I harbors a novel peroxisomal targeting signal 1 and is essential for flowering and development. Planta, 251. | en_US |
dc.identifier.issn | 0032-0935 | |
dc.identifier.uri | https://hdl.handle.net/11250/2754993 | |
dc.description.abstract | A putative, non-canonical peroxisomal targeting signal type 1 (PTS1) Pro-Arg-Met > was identified in the extreme C-terminus of trehalose-6-phosphate phosphatase (TPP)I. TPP catalyzes the final step of trehalose synthesis, and the enzyme was previously characterized to be nuclear only (Krasensky et al. in Antioxid Redox Signal 21(9):1289–1304, 2014). Here we show that the TPPI C-terminal decapeptide ending with Pro-Arg-Met > or Pro-Lys-Met > can indeed function as a PTS1. Upon transient expression in two plant expression systems, the free C- or N-terminal end led to the full-length TPPI targeting to peroxisomes and plastids, respectively. The nucleus and nucleolus targeting of the full-length TPPI was observed in both cases. The homozygous T-DNA insertion line of TPPI showed a pleiotropic phenotype including smaller leaves, shorter roots, delayed flowering, hypersensitivity to salt, and a sucrose dependent seedling development. Our results identify novel PTS1s, and TPPI as a protein multi-targeted to peroxisomes, plastids, nucleus, and nucleolus. Altogether our findings implicate an essential role for TPPI in development, reproduction, and cell signaling. | en_US |
dc.language.iso | eng | en_US |
dc.publisher | Springer Nature Switzerland AG | en_US |
dc.rights | Navngivelse 4.0 Internasjonal | * |
dc.rights.uri | http://creativecommons.org/licenses/by/4.0/deed.no | * |
dc.subject | phosphorylation | en_US |
dc.subject | PTS1 | en_US |
dc.title | Multi-targeted trehalose-6-phosphate phosphatase I harbors a novel peroxisomal targeting signal 1 and is essential for flowering and development | en_US |
dc.type | Peer reviewed | en_US |
dc.type | Journal article | en_US |
dc.description.version | publishedVersion | en_US |
dc.rights.holder | © The Author(s) 2020 | en_US |
dc.subject.nsi | VDP::Matematikk og Naturvitenskap: 400::Basale biofag: 470 | en_US |
dc.source.volume | 251 | en_US |
dc.source.journal | Planta | en_US |
dc.source.issue | 5 | en_US |
dc.identifier.doi | 10.1007/s00425-020-03389-z | |
dc.identifier.cristin | 1886025 | |
dc.source.articlenumber | 98 (2020) | en_US |
cristin.ispublished | true | |
cristin.fulltext | original | |
cristin.qualitycode | 2 | |