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dc.contributor.advisorSydnes, Magne Olav
dc.contributor.advisorPampanin, Daniela Maria
dc.contributor.authorVaaland, Ingrid Caroline
dc.date.accessioned2019-01-15T09:57:56Z
dc.date.available2019-01-15T09:57:56Z
dc.date.issued2018-09
dc.identifier.urihttp://hdl.handle.net/11250/2580632
dc.descriptionMaster's thesis in Environmental Technologynb_NO
dc.description.abstractThe currently used GC-MS method for analysis of PAH metabolites in fish bile only focus on phenols; 1-naphthol, 2-naphthol, 1-hydroxyphenanthrene, and alkylated derivatives. 1- Hydroxypyrene is also included, but not alkylated forms. However, since trans-naphthalene dihydrodiol and methylated derivatives are formed in vivo and are known carcinogens, their presence in fish bile should be further investigated. Due to the absence of commercially available standards of trans-dihydro diols and their methylated derivatives, these compounds need to be synthesized. trans-1,2-Dihydronaphthalene-1,2-diol (13±), trans-6-methyl-1,2-dihydronaphthalene-1,2- diol (19±), trans-5,7-dimethyl-1,2-dihydronaphthalene-1,2-diol (20±) and trans-4,6,7- trimethyl-1,2-dihydronaphthalene-1,2-diol (21±) were synthesized from commercially available starting materials, and their overall yield were 40%, 59%, 28% and 3%, respectively. The compunds were prepared from 1,2-naphthoquinone, 6-methyl-1-tetralone, 5,7-dimethyl-1- tetralone, 1,2-dihydroxybenzene and 2,3-dimethyl-1,3-butadiene, and oxidation was performed by using 2-iodoxybenzoic acid (IBX) or Laccase-catalyzed Diels-Alder reaction, following reduction by sodium borohydride (NaBH4) to give the trans-naphthalene dihydrodiols. Fish bile samples obtained from Atlantic cod (Gadus morhua), exposed to dispersed crude oil, were prepared by de-conjugation, extraction and derivatisation, following standard procedure in order to prepare the samples for GC-MS analysis.nb_NO
dc.language.isoengnb_NO
dc.publisherUniversity of Stavanger, Norwaynb_NO
dc.relation.ispartofseriesMasteroppgave/UIS-TN-IKBM/2018;
dc.subjectLaccase Trametes versicolornb_NO
dc.subjectatlanterhavstorsknb_NO
dc.subjectGadus morhuanb_NO
dc.subjectorganic synthesisnb_NO
dc.subjectbiliary metabolitesnb_NO
dc.subjectnaphthalene trans-dihydrodiolsnb_NO
dc.subjectteknisk miljøvernnb_NO
dc.titleNaphthalene trans-dihydrodiolsnb_NO
dc.typeMaster thesisnb_NO
dc.subject.nsiVDP::Teknologi: 500::Miljøteknologi: 610nb_NO


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