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dc.contributor.authorVerheyen, Davy
dc.contributor.authorXu, Xiang Ming
dc.contributor.authorGovaert, Marlies
dc.contributor.authorBaka, Maria
dc.contributor.authorSkåra, Torstein
dc.contributor.authorVan Impe, Jan FM
dc.date.accessioned2019-12-23T12:32:17Z
dc.date.available2019-12-23T12:32:17Z
dc.date.created2019-09-05T09:28:21Z
dc.date.issued2019-06
dc.identifier.citationVerheyen, D., Ming X, X., Govaerta, M. et al. (2019) Food Microstructure and Fat Content Affect Growth Morphology, Growth Kinetics, and Preferred Phase for Cell Growth of Listeria monocytogenes in Fish-Based Model Systems. Applied and Environmental Microbiology, DOI: 10.1128/AEM.00707-19nb_NO
dc.identifier.issn0099-2240
dc.identifier.urihttp://hdl.handle.net/11250/2634409
dc.description.abstractFood microstructure significantly affects microbial growth dynamics, but knowledge concerning the exact influencing mechanisms at a microscopic scale is limited. The food microstructural influence on Listeria monocytogenes (green fluorescent protein strain) growth at 10°C in fish-based food model systems was investigated by confocal laser scanning microscopy. The model systems had different microstructures, i.e., liquid, xanthan (high-viscosity liquid), aqueous gel, and emulsion and gelled emulsion systems varying in fat content. Bacteria grew as single cells, small aggregates, and microcolonies of different sizes (based on colony radii [size I, 1.5 to 5.0 μm; size II, 5.0 to 10.0 μm; size III, 10.0 to 15.0 μm; and size IV, ≥15 μm]). In the liquid, small aggregates and size I microcolonies were predominantly present, while size II and III microcolonies were predominant in the xanthan and aqueous gel. Cells in the emulsions and gelled emulsions grew in the aqueous phase and on the fat-water interface. A microbial adhesion to solvent assay demonstrated limited bacterial nonpolar solvent affinities, implying that this behavior was probably not caused by cell surface hydrophobicity. In systems containing 1 and 5% fat, the largest cell volume was mainly represented by size I and II microcolonies, while at 10 and 20% fat a few size IV microcolonies comprised nearly the total cell volume. Microscopic results (concerning, e.g., growth morphology, microcolony size, intercolony distances, and the preferred phase for growth) were related to previously obtained macroscopic growth dynamics in the model systems for an L. monocytogenes strain cocktail, leading to more substantiated explanations for the influence of food microstructural aspects on lag phase duration and growth rate. IMPORTANCE Listeria monocytogenes is one of the most hazardous foodborne pathogens due to the high fatality rate of the disease (i.e., listeriosis). In this study, the growth behavior of L. monocytogenes was investigated at a microscopic scale in food model systems that mimic processed fish products (e.g., fish paté and fish soup), and the results were related to macroscopic growth parameters. Many studies have previously focused on the food microstructural influence on microbial growth. The novelty of this work lies in (i) the microscopic investigation of products with a complex composition and/or structure using confocal laser scanning microscopy and (ii) the direct link to the macroscopic level. Growth behavior (i.e., concerning bacterial growth morphology and preferred phase for growth) was more complex than assumed in common macroscopic studies. Consequently, the effectiveness of industrial antimicrobial food preservation technologies (e.g., thermal processing) might be overestimated for certain products, which may have critical food safety implications.nb_NO
dc.language.isoengnb_NO
dc.publisherAmerican Society for Microbiologynb_NO
dc.subjectmikrobiologinb_NO
dc.titleFood Microstructure and Fat Content Affect Growth Morphology, Growth Kinetics, and Preferred Phase for Cell Growth of Listeria monocytogenes in Fish-Based Model Systemsnb_NO
dc.typeJournal articlenb_NO
dc.typePeer reviewednb_NO
dc.description.versionacceptedVersionnb_NO
dc.rights.holderCopyright © 2019 American Society for Microbiology.nb_NO
dc.subject.nsiVDP::Mathematics and natural science: 400::Basic biosciences: 470::General microbiology: 472nb_NO
dc.source.volume85nb_NO
dc.source.journalApplied and Environmental Microbiologynb_NO
dc.source.issue16nb_NO
dc.identifier.doi10.1128/AEM.00707-19
dc.identifier.cristin1721746
cristin.unitcode217,8,10,1
cristin.unitnameLaboratorium IKBM
cristin.ispublishedtrue
cristin.fulltextpostprint
cristin.qualitycode2


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