Protein phosphatase 4 recombinant protein – transformation of bacteria and Arabidopsis thaliana
Master thesis
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http://hdl.handle.net/11250/2410603Utgivelsesdato
2016-06Metadata
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Sammendrag
Protein phosphatase 4 (PP4) is well conserved in eukaryote organisms. The protein has been
studied in yeast and
mammalian cells, but little is known about PP4 in plants. The function of
PP4 in plants is unknown and it will not have the same function
as in
the other studied
organisms because the processes does not exist in plants. It is likely that the PP4 proteins has
been adapted for other uses in plants.
In this thesis project, I worked toward making
Arabidopsis thaliana
plants with the two PP4
proteins PP4-1 and PP4-2 using a variety of recombination technologies. Plants were
successfully transformed with PP4-2 and PP4-1. The plants with PP4-2 made seeds that have
a chance of having the PP4-2 gene themselves. These plants
can be used for further study of
PP4-2 in plants.
As the practical part of the thesis work ended, the plants transformed with the PP4-1 gene
were starting to produce seeds that later can be selected for the gene and grow to produce
more seeds.
These can potentially
be used in further studies.
I also demonstrated that the PP4-2 protein could be produced with
E. coli
Rosetta strain. The
protein was visualized on a polyacrylamide gel.
The PP4-1
has been successfully cloned into a pMAL-C2X vector that can
be used for further
studies.
Beskrivelse
Master's thesis in Biological chemistry