dc.contributor.author | Are, Tina Marie Monge | |
dc.date.accessioned | 2015-01-07T14:52:29Z | |
dc.date.available | 2015-01-07T14:52:29Z | |
dc.date.issued | 2014-06-16 | |
dc.identifier.uri | http://hdl.handle.net/11250/273719 | |
dc.description | Master's thesis in Biological chemistry | nb_NO |
dc.description.abstract | Cervical cancer is ranked as the fourth most common cancer worldwide, and the second leading cause of mortality among young woman 19 – 39 of age. Cervical cancer arises from persistent human papilloma (HPV) infection and may take several years to develop. Because of organized screening a premalignant condition of the disease, Cervical Intraepithelial Neoplasia (CIN), can be detected and treated before it becomes invasive and metastatic. microRNA (miRNA) are defined as a class of small non-coding regulatory RNAs, approximately 22 nucleotides long. The miRNAs interferes with the post-transcriptional regulation of gene expression by base-pairing with the 3`-untranslated region of target messenger RNA. MiR-18a is a member of the miR-17-92 cluster which has been found to be a modulator of effector and memory T-cells. In addition, miR-18a and miR-18b have been found to play a role in development of estrogen receptor alpha (ER-α) negative breast cancer.
The aim of this thesis was to optimize methods for isolation, purification and detection of miR-18a and miR-18b in FFPE cervical specimens. The expression of miR-18a and -18b in persistent HPV-16 positive CIN3 samples and normal cervical samples were compared by the use of RT-qPCR and semi-quantitative scoring with CISH.
We found that miR-18a and miR-18b were highly expressed in CIN3 lesions as compared to normal cervical tissue. This might show that high expression of these miRNAs, is a sign of poor prognosis of a lesion with potential to developing into cancer. A comparison between CD8+ T-cells and miR-18a expression indicated a possible relationship between these cytotoxic T-cells and and the miR-18a. | nb_NO |
dc.language.iso | eng | nb_NO |
dc.publisher | University of Stavanger, Norway | nb_NO |
dc.rights | Attribution-NonCommercial 3.0 Norway | * |
dc.rights.uri | http://creativecommons.org/licenses/by-nc/3.0/no/ | * |
dc.subject | kreft | nb_NO |
dc.subject | cervical cancer | nb_NO |
dc.subject | biologisk kjemi | nb_NO |
dc.subject | HPV | nb_NO |
dc.subject | humant papillomavirus | nb_NO |
dc.subject | livmorhalskreft | |
dc.title | Expression of microRNA-18a and microRNA-18b in the microenvironment of high-grade Cervical Intraepithelial Neoplasia (CIN2-3) | nb_NO |
dc.type | Master thesis | nb_NO |
dc.subject.nsi | VDP::Mathematics and natural science: 400::Basic biosciences: 470::Molecular biology: 473 | nb_NO |
dc.subject.nsi | VDP::Mathematics and natural science: 400::Chemistry: 440::Organic chemistry: 441 | nb_NO |