Lil3 dimerization and chlorophyll binding in Arabidopsis thaliana

Mork-Jansson, Astrid Elisabeth; Gargano, Daniela; Kmiec, Karol; Furnes, Clemens; Shevela, Dmitriy; Eichacker, Lutz
Peer reviewed, Journal article
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https://hdl.handle.net/11250/3039146
Date
2015
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Mork-Jansson, A. E., Gargano, D., Kmiec, K., Furnes, C., Shevela, D., & Eichacker, L. A. (2015). Lil3 dimerization and chlorophyll binding in Arabidopsis thaliana. FEBS letters, 589(20), 3064-3070.   10.1016/j.febslet.2015.08.023
Abstract
The two-helix light harvesting like (Lil) protein Lil3 belongs to the family of chlorophyll binding light harvesting proteins of photosynthetic membranes. A function in tetrapyrrol synthesis and stabilization of geranylgeraniol reductase has been shown. Lil proteins contain the chlorophyll a/b-binding motif; however, binding of chlorophyll has not been demonstrated. We find that Lil3.2 fromArabidopsis thaliana forms heterodimers with Lil3.1 and binds chlorophyll. Lil3.2 heterodimerization (25 ± 7.8 nM) is favored relative to homodimerization (431 ± 59 nM). Interaction of Lil3.2 with chlorophyll a (231 ± 49 nM) suggests that heterodimerization precedes binding of chlorophyll inArabidopsis thaliana.
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FEBS Letters
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The authors