Enrichment of immunoaffinity technique to capture Lil3 proteins
Master thesis
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http://hdl.handle.net/11250/182535Utgivelsesdato
2012Metadata
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Sammendrag
The experiment in this thesis is set out to learn and understand the biology or chemistry basis
and to apply different techniques used in protein biochemistry research as well as to observe
the results in practice. Among the various methods of studying proteins, affinity method is
regarded as one of the most effective means of purifying proteins as a result of its high degree
of specificity. This experiment has demonstrated how immunoaffinity technique can be used
to capture Lil3 proteins from a crude source of solubilized thylakoid membrane.
Immobilization method by coupling Lil3 antibody directly onto Toyopearls beads support
was performed and the coupling efficiency was evaluated. The Lil3 proteins captured by
antibody column were eluted with denaturing and nondenaturing elution buffers.
Regeneration and reuse of the immobilized antibody-coupled beads column have been
conducted for a few times, in an attempt to conserve the limited supply antibody and the
economic feasibility, including time and expense. In addition, a brief analysis of protein
membrane complexes and antibody was also provided.
Beskrivelse
Master's thesis in Biological chemistry