A strategy to characterize chlorophyll protein interaction in LIL3
Peer reviewed, Journal article
Published version
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https://hdl.handle.net/11250/3046332Utgivelsesdato
2019Metadata
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Originalversjon
Mork-Jansson, A. E., & Eichacker, L. A. (2019). A strategy to characterize chlorophyll protein interaction in LIL3. Plant Methods, 15(1), 1-13. 10.1186/s13007-018-0385-5Sammendrag
The function of proteins is at large determined by cofactors selectively bound to protein structure. Without chlorophyll specifically bound to protein, light harvesting and photosynthesis would not be possible. The binding of chlorophyll to light harvesting proteins has been extensively studied in reconstitution assays using proteins expressed in vitro; however, the mechanism of the reconstitution reaction remained unclear. We have shown that membrane integral light-harvesting-like protein, LIL3, binds chlorophyll a with a Kd of 146 nM in vitro by thermophoresis. Here, reconstitution of chlorophyll binding to LIL3 has been characterized by four different methods.