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dc.contributor.authorL'Heureux, Joanna E.
dc.contributor.authorvan der Giezen, Mark
dc.contributor.authorWinyard, Paul G.
dc.contributor.authorJones, Andrew Michael
dc.contributor.authorVanhatalo, Anni
dc.date.accessioned2024-07-08T12:28:25Z
dc.date.available2024-07-08T12:28:25Z
dc.date.created2024-01-09T15:58:33Z
dc.date.issued2023-12
dc.identifier.citationL’Heureux, J. E., van der Giezen, M., Winyard, P. G., Jones, A. M., & Vanhatalo, A. (2023). Localisation of nitrate-reducing and highly abundant microbial communities in the oral cavity. Plos one, 18(12), e0295058.en_US
dc.identifier.issn1932-6203
dc.identifier.urihttps://hdl.handle.net/11250/3139303
dc.description.abstractThe nitrate (NO3-) reducing bacteria resident in the oral cavity have been implicated as key mediators of nitric oxide (NO) homeostasis and human health. NO3--reducing oral bacteria reduce inorganic dietary NO3- to nitrite (NO2-) via the NO3--NO2--NO pathway. Studies of oral NO3--reducing bacteria have typically sampled from either the tongue surface or saliva. The aim of this study was to assess whether other areas in the mouth could contain a physiologically relevant abundance of NO3- reducing bacteria, which may be important for sampling in clinical studies. The bacterial composition of seven oral sample types from 300 individuals were compared using a meta-analysis of the Human Microbiome Project data. This analysis revealed significant differences in the proportions of 20 well-established oral bacteria and highly abundant NO3--reducing bacteria across each oral site. The genera included Actinomyces, Brevibacillus, Campylobacter, Capnocytophaga, Corynebacterium, Eikenella, Fusobacterium, Granulicatella, Haemophilus, Leptotrichia, Microbacterium, Neisseria, Porphyromonas, Prevotella, Propionibacterium, Rothia, Selenomonas, Staphylococcus, Streptococcus and Veillonella. The highest proportion of NO3--reducing bacteria was observed in saliva, where eight of the bacterial genera were found in higher proportion than on the tongue dorsum, whilst the lowest proportions were found in the hard oral surfaces. Saliva also demonstrated higher intra-individual variability and bacterial diversity. This study provides new information on where samples should be taken in the oral cavity to assess the abundance of NO3--reducing bacteria. Taking saliva samples may benefit physiological studies, as saliva contained the highest abundance of NO3- reducing bacteria and is less invasive than other sampling methods. These results inform future studies coupling oral NO3--reducing bacteria research with physiological outcomes affecting human health.en_US
dc.language.isoengen_US
dc.publisherPublic Library of Science (PLoS)en_US
dc.rightsNavngivelse 4.0 Internasjonal*
dc.rights.urihttp://creativecommons.org/licenses/by/4.0/deed.no*
dc.titleLocalisation of nitrate-reducing and highly abundant microbial communities in the oral cavityen_US
dc.typePeer revieweden_US
dc.typeJournal articleen_US
dc.description.versionpublishedVersionen_US
dc.rights.holder© 2023 L’Heureux et al.en_US
dc.subject.nsiVDP::Matematikk og Naturvitenskap: 400::Basale biofag: 470en_US
dc.source.volume18en_US
dc.source.journalPLOS ONEen_US
dc.source.issue12en_US
dc.identifier.doi10.1371/journal.pone.0295058
dc.identifier.cristin2223436
dc.source.articlenumbere0295058en_US
cristin.ispublishedtrue
cristin.fulltextoriginal
cristin.qualitycode1


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