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dc.contributor.advisorKommedal, Roald
dc.contributor.authorHåland, Mari Wøien
dc.date.accessioned2019-10-22T09:18:09Z
dc.date.available2019-10-22T09:18:09Z
dc.date.issued2019-06
dc.identifier.urihttp://hdl.handle.net/11250/2623658
dc.descriptionMaster's thesis in Environmental engineeringnb_NO
dc.description.abstractThe presence of antibiotics in wastewater treatment plants is a concern, both because of potential release to natural water systems, and connected to the spread of antibiotic resistance genes. In this thesis, the focus is on the β-lactam antibiotic ampicillin in activated sludge. A method for analysing ampicillin in wastewater samples was found in literature, adapted, and tested using concentrations in the 0.050-1.0 mg/L range. Key steps in the method are sample preparation by solid phase extraction (SPE), and analysis by high performance liquid chromatography (HPLC) with UV-detection. Automated SPE by a RapidTrace® instrument was employed. β-lactam antibiotics cloxacillin and dicloxacillin were used as internal standards. When applied, the method measured ampicillin concentrations down to 10 μg/L. Recovery from SPE on wastewater effluent samples was found to be 75% or greater, with high precision. A version of the method for larger sample sizes proved time-consuming. The developed method was used to study the fate of ampicillin in activated sludge, with a focus on adsorption. This included measuring removal over time and an attempt at equilibrium modelling using Freundlich and Langmuir adsorption isotherms. The results from these studies were largely inconclusive. However, removal of ampicillin in the sludge was found to be high, for 1-3 g/L mixed liquor suspended solids (MLSS). Some of the removal was shown to be due to chemical degradation.nb_NO
dc.language.isoengnb_NO
dc.publisherUniversity of Stavanger, Norwaynb_NO
dc.relation.ispartofseriesMasteroppgave/UIS-TN-IKBM/2019;
dc.rightsAttribution-NonCommercial-NoDerivatives 4.0 Internasjonal*
dc.rights.urihttp://creativecommons.org/licenses/by-nc-nd/4.0/deed.no*
dc.subjectadsorptionnb_NO
dc.subjectactivated sludgenb_NO
dc.subjectantibioticsnb_NO
dc.subjectampicillinnb_NO
dc.subjectβ-lactamnb_NO
dc.subjecthigh performance liquid chromatographynb_NO
dc.subjectsequencing batch reactornb_NO
dc.subjectsolid phase extractionnb_NO
dc.subjectteknisk miljøvernnb_NO
dc.subjectvannnb_NO
dc.subjectenvironmental technologynb_NO
dc.subjectwater science and technologynb_NO
dc.subjectmiljøteknologinb_NO
dc.subjectantibiotikanb_NO
dc.titleAnalysis of the Antibiotic Ampicillin in Activated Sludge: Method Development and Adsorption Studiesnb_NO
dc.typeMaster thesisnb_NO
dc.subject.nsiVDP::Teknologi: 500::Miljøteknologi: 610nb_NO


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Attribution-NonCommercial-NoDerivatives 4.0 Internasjonal
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