| dc.description.abstract | Cultivation of medicinal plants under control conditions in greenhouses can be promising technology for supplying pharmaceuticals. The main challenge of growing medicinal plants is that we have to create environmental conditions which allow plants growth with high rate and accumulate high amount of biologically active compounds. To address this challenge, we used three approaches. First, we compared different medicinal species under hydroponics and aeroponics conditions; second, we estimated the effect of ammonium and third, we estimated the effect of plant inoculation with R.rhizogenes. We found that different medicinal species have high variation in plant growth rate, however, plant growth was not dependent of accumulation of biologically active compounds (total phenolics). This indicates, that it is possible to select species which combine both high relative growth and high accumulation of biologically active compounds. From 5 tested species, the most interesting species for farther investigation is H.perforatum. Aeroponics induced strong formation of root hairs, however in most case, it does not increase accumulation of biologically active compounds, indicating no advantages of aeroponcis compare with hydroponics for cultivation of medicinal plants. Most important, ammonium supply applied for plants cultivated in aeroponics induced plant growth inhibition, however, ammonium has no or week negative effect for plants grown hydroponically. As ammonium was able increase accumulation of secondary compounds, this treatment is promising for enhancement of accumulation of biologically active compounds in medicinal plants. Inoculation with A.rhizogenes does not increase concentration of biologically active compounds in Artemisiad, however the specific strain 18834 strongly enhace artemisinin amaoaunt in root exudates indicated the possible regulation of artemisin transport. In common, cultivation of medic | nb_NO |
