| dc.description.abstract | Keywords: Oxford Nanopore sequencing, 16S rRNA gene sequencing, IBD
Since IBD is an increasing disease globally, it is vital to find out more about it. IBD causes many symptoms for the patient, including somatic symptoms and mental issues.
A correlation between gut microbiota and IBD has been acknowledged in the last decade. IBD patients have increased dysbiosis caused by bacteria present in the gut. Bioinformatics is an increasing field, where there are more and more genomic databases able to identify microbiota both cheaper and faster than before.
The University of Stavanger has a large project sequencing stool samples in cooperation with SUS. The faecal matter is extracted before identifying bacterial findings. The stool samples are collected from the hospital in Stavanger (SUS) of patients with CD or UC, where the goal is to understand the microbiome better.
The bacterial 16S rRNA genes DNA were extracted and amplified. DNA was sequenced with Oxford Nanopore Sequencing before the data was processed. The bacterial findings showed decreased diversity in phyla in bacteria with IBD compared to the control and the dog.
The result indicates that the Firmicutes and Bacteroidetes ratio is neither valuable for treatment responses nor an IBD disease marker. The phyla diversity differed from the control, and one sample had an increasing diversity trend after treating the same patient. | |