Exploring potential analytical methods for determination of glycerol-3-phosphate in human plasma
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Chronic kidney disease (CKD) is a progressive condition where the kidneys gradually lose their ability to function properly. Biomarkers that indicate immediate renal tissue injury is needed for improved detection of early CKD. One potential biomarker is glycerol-3-phosphate (G3P), a small organic ion which recently has been inked to kidney injury through regulation of fibroblast growth factor 23 (FGF23). The aim of this study was to explore potential analytical methods for determination of G3P in human plasma. G3P was retained and analyzed on a hydrophilic interaction liquid chromatography (HILIC) column using liquid chromatography-mass spectrometry (LC-MS). Further, derivatization with alcohols through an esterification reaction was possible, which offered retention using reversed phase (RP) LC. However, the reaction conditions potentially lead to phospholipid hydrolysis, which increased the amount of G3P. Further research could explore the possibilities of phospholipid extraction through liquid-liquid extraction (LLE) or solid phase extraction (SPE).