Vekstvurdering av pankreatisk duktalt adenokarsinom Panc-1 cellelinje i kollagen og alginat med forskjellige mekaniske egenskaper.

Abstract

This thesis explored the effects of varying concentrations of collagen and alginate on the growth of the Panc-1 pancreatic cancer cell line. The research employs 3D culturing techniques to simulate the tumor microenvironment, using collagen and alginate as substrates to modulate the physical stress exerted on the cells. By adjusting the concentrations of these biopolymers, the study replicates various stiffness levels that mimic the natural stiffness found within the human body. The primary methods utilized include rheological measurements to determine the mechanical properties of the alginate gels and resazurin assays to assess cell viability.

Results indicate that Panc-1 cells exhibit distinct proliferative behaviors in response to the different mechanical stresses induced by the biopolymer matrices. Cells cultured in lower alginate concentrations demonstrated enhanced proliferation and viability, suggesting that softer matrices are more conducive to cell growth. In contrast, the response of cells in collagen matrices was more complex, with varying effects on cell proliferation observed across different stiffness levels.

The findings highlight the potential of manipulating ECM properties to influence cancer cell behavior and suggest that altering matrix stiffness could be a viable approach to understand more of how the stroma affects cancer cells in pancreatic cancer.

This thesis explored the effects of varying concentrations of collagen and alginate on the growth of the Panc-1 pancreatic cancer cell line. The research employs 3D culturing techniques to simulate the tumor microenvironment, using collagen and alginate as substrates to modulate the physical stress exerted on the cells. By adjusting the concentrations of these biopolymers, the study replicates various stiffness levels that mimic the natural stiffness found within the human body. The primary methods utilized include rheological measurements to determine the mechanical properties of the alginate gels and resazurin assays to assess cell viability.

Results indicate that Panc-1 cells exhibit distinct proliferative behaviors in response to the different mechanical stresses induced by the biopolymer matrices. Cells cultured in lower alginate concentrations demonstrated enhanced proliferation and viability, suggesting that softer matrices are more conducive to cell growth. In contrast, the response of cells in collagen matrices was more complex, with varying effects on cell proliferation observed across different stiffness levels.

The findings highlight the potential of manipulating ECM properties to influence cancer cell behavior and suggest that altering matrix stiffness could be a viable approach to understand more of how the stroma affects cancer cells in pancreatic cancer.